| Saturday 19th August 2000 | First Division | ||
|---|---|---|---|
| Stone Dominoes | 4-0 | Holker Old Boys | |
| Tuesday 22nd August 2000 | First Division | ||
|---|---|---|---|
| Holker Old Boys | 0-6 | Blackpool Mechanics | |
| Saturday 26th August 2000 | First Division | ||
|---|---|---|---|
| Holker Old Boys | 6-0 | Aylestone Park | |
| Monday 28th August 2000 | First Division | ||
|---|---|---|---|
| Bootle | 2-1 | Holker Old Boys | |
| Saturday 2nd September 2000 | First Division | ||
|---|---|---|---|
| Daisy Hill | 4-1 | Holker Old Boys | |
| Saturday 9th September 2000 | First Division | ||
|---|---|---|---|
| Holker Old Boys | 1-0 | Atherton LR | |
| Saturday 16th September 2000 | First Division | ||
|---|---|---|---|
| Holker Old Boys | 5-0 | Oldham Town | |
| Saturday 23rd September 2000 | F.A. Carlsberg Vase 2nd Qualifying Round | ||
|---|---|---|---|
| Northallerton Town | 0-2 | Holker Old Boys | Att: 43 |
|
Carl Waters (60) Graham Cotterill (79) | |||
| Saturday 30th September 2000 | First Division | ||
|---|---|---|---|
| Holker Old Boys | 2-0 | Castleton Gabriels | |
| Saturday 14th October 2000 | League Challenge Cup Preliminary Round | ||
|---|---|---|---|
| Formby | 1-0 | Holker Old Boys | Att: 46 |
| Carl Dale (41) | |||
| Tuesday 17th October 2000 | First Division | ||
|---|---|---|---|
| Castleton Gabriels | 3-1 | Holker Old Boys | |
| Saturday 21st October 2000 | F.A. Carlsberg Vase 1st Round | ||
|---|---|---|---|
| Clitheroe | 2-0 | Holker Old Boys | Att: 169 |
|
Barry Hart (38p) Barry Hart (40p) |
|||
| Tuesday 26th December 2000 | First Division | ||
|---|---|---|---|
| Holker Old Boys | 3-5 | Darwen | |
| Saturday 6th January 2001 | First Division | ||
|---|---|---|---|
| Holker Old Boys | 1-3 | Daisy Hill | |
| Saturday 13th January 2001 | First Division | ||
|---|---|---|---|
| Chadderton | 3-0 | Holker Old Boys | |
| Saturday 27th January 2001 | First Division | ||
|---|---|---|---|
| Warrington Town | 2-0 | Holker Old Boys | |
| Tuesday 30th January 2001 | First Division | ||
|---|---|---|---|
| Blackpool Mechanics | 2-2 | Holker Old Boys | |
| Tuesday 6th February 2001 | First Division | ||
|---|---|---|---|
| Nelson | 1-0 | Holker Old Boys | |
| Saturday 10th February 2001 | First Division | ||
|---|---|---|---|
| Tetley Walker | 4-1 | Holker Old Boys | |
| Saturday 17th February 2001 | First Division | ||
|---|---|---|---|
| Colne | 0-1 | Holker Old Boys | |
| Tuesday 20th February 2001 | First Division | ||
|---|---|---|---|
| Holker Old Boys | 2-2 | Padiham | |
| Saturday 24th February 2001 | First Division | ||
|---|---|---|---|
| Holker Old Boys | 2-0 | Stone Dominoes | |
| Saturday 3rd March 2001 | First Division | ||
|---|---|---|---|
| Holker Old Boys | 4-0 | Ashton Town | |
| Tuesday 6th March 2001 | First Division | ||
|---|---|---|---|
| Holker Old Boys | 3-3 | Bootle | |
| Saturday 10th March 2001 | First Division | ||
|---|---|---|---|
| Aylestone Park | 2-1 | Holker Old Boys | |
| Tuesday 13th March 2001 | First Division | ||
|---|---|---|---|
| Holker Old Boys | 3-2 | Nelson | |
| Saturday 17th March 2001 | First Division | ||
|---|---|---|---|
| Atherton LR | 1-0 | Holker Old Boys | |
| Tuesday 20th March 2001 | First Division | ||
|---|---|---|---|
| Holker Old Boys | 2-0 | Squires Gate | |
| Saturday 24th March 2001 | First Division | ||
|---|---|---|---|
| Holker Old Boys | 2-1 | Colne | |
| Tuesday 3rd April 2001 | First Division | ||
|---|---|---|---|
| Holker Old Boys | 2-3 | Chadderton | |
| Saturday 14th April 2001 | First Division | ||
|---|---|---|---|
| Holker Old Boys | 3-1 | Warrington Town | |
| Monday 16th April 2001 | First Division | ||
|---|---|---|---|
| Formby | 2-2 | Holker Old Boys | |
| Wednesday 18th April 2001 | First Division | ||
|---|---|---|---|
| Holker Old Boys | 0-0 | Bacup Borough | |
| Saturday 21st April 2001 | First Division | ||
|---|---|---|---|
| Holker Old Boys | 2-3 | Tetley Walker | |
| Tuesday 24th April 2001 | First Division | ||
|---|---|---|---|
| Darwen | 7-3 | Holker Old Boys | |
| Thursday 26th April 2001 | First Division | ||
|---|---|---|---|
| Bacup Borough | 3-0 | Holker Old Boys | |
| Saturday 28th April 2001 | First Division | ||
|---|---|---|---|
| Padiham | 1-2 | Holker Old Boys | |
| Tuesday 1st May 2001 | First Division | ||
|---|---|---|---|
| Squires Gate | 2-1 | Holker Old Boys | |
| Saturday 5th May 2001 | First Division | ||
|---|---|---|---|
| Oldham Town | 3-1 | Holker Old Boys | |
| Monday 7th May 2001 | First Division | ||
|---|---|---|---|
| Holker Old Boys | 2-1 | Formby | |
| Saturday 12th May 2001 | First Division | ||
|---|---|---|---|
| Ashton Town | 3-5 | Holker Old Boys | |
