| Sat 13/09/25 | Steeton v Darwen FC * | 1-2 | | | | | | |
| Sat 27/09/25 | Darwen FC v Garstang (CUP) | 0-0 | |  | | | | |
| Sat 04/10/25 | Darwen FC v Maghull | 5-2 | | | |  | |  |
| Sat 11/10/25 | Daisy Hill v Darwen FC | 3-2 | | | | | | |
| Sat 25/10/25 | Darwen FC v Garstang | 4-0 | | | | | | |
| Wed 29/10/25 | Darwen FC v Colne (CUP) | 4-0 | | | | | | |
| Sat 01/11/25 | Darwen FC v Ashton Athletic | 3-1 | | | | | | |
| Sat 08/11/25 | Colne v Darwen FC | 4-1 | | | | | | |
| Sat 15/11/25 | Ashton Town v Darwen FC (CUP) | 2-3 | | | | | | |
| Wed 19/11/25 | Darwen FC v Bacup Borough | 1-1 | | | | |  | |
| Sat 22/11/25 | Maghull v Darwen FC | 1-1 | | | | | | |
| Sat 13/12/25 | Darwen FC v Holker Old Boys | 3-3 | | | | | | |
| Fri 26/12/25 | Darwen FC v Nelson | 0-2 | | | | | | |
| Sat 10/01/26 | Darwen FC v Droylsden * | 2-3 | | | | | | |
| Sat 17/01/26 | Ashton Town v Darwen FC | 2-0 | | | | | | |
| Tue 20/01/26 | Fulwood Amateurs v Darwen FC | 2-2 | | | | | | |
| Sat 24/01/26 | Darwen FC v Maine Road | 2-1 | | | | | | |
| Fri 30/01/26 | Barnton v Darwen FC (CUP) | 2-2 | | | | | | |
| Wed 04/02/26 | Bacup Borough v Darwen FC | 1-1 | | | | | | |
| Sat 07/02/26 | Thornton Cleveleys v Darwen FC | 3-7 | | | | | | |
| Sat 14/02/26 | Darwen FC v Daisy Hill | 3-2 | | | | | | |
| Sat 21/02/26 | Maine Road v Darwen FC | 0-1 | | | | | | |
| Fri 27/02/26 | Darwen FC v Colne | 0-0 | | | | | | |
| Sat 07/03/26 | Garstang v Darwen FC | 2-1 | | | | | | |
| Sat 14/03/26 | Darwen FC v Stockport Georgians (CUP) | 1-0 | | | | | | |
| Sat 21/03/26 | AFC Blackpool v Darwen FC | 1-2 | | | | | | |
| Wed 25/03/26 | Darwen FC v Steeton | 2-0 | | | | | | |
| Sat 28/03/26 | MSB Woolton v Darwen FC | 2-1 | | | | | | |
| Sat 04/04/26 | Darwen FC v Squires Gate | 2-1 | | | | | | |
| Mon 06/04/26 | Nelson v Darwen FC | 1-0 | | | | | | |
