| Sat 26/07/25 | Ramsbottom United v Atherton LR | 2-1 | | | | | | |
| Tue 29/07/25 | Atherton LR v South Liverpool * | 1-3 | | | | | | |
| Sat 02/08/25 | Atherton LR v Ossett United (CUP) * | 1-0 | |  | | | | |
| Sat 09/08/25 | City of Liverpool v Atherton LR * | 0-6 |  | | | | | |
| Wed 13/08/25 | West Didsbury & Chorlton v Atherton LR * | 0-1 | | | | | | |
| Sat 16/08/25 | FC St Helens v Atherton LR (CUP) * | 0-2 | | | | | | |
| Mon 25/08/25 | Atherton LR v Stockport Town | 5-0 | | | | | |  |
| Sat 06/09/25 | Cheadle Town v Atherton LR * | 1-3 | | | | | | |
| Tue 09/09/25 | Atherton LR v Chadderton | 0-1 | | | | | | |
| Sat 13/09/25 | Macclesfield v Atherton LR (CUP) * | 3-0 | | | | | | |
| Tue 16/09/25 | Atherton LR v Litherland REMYCA * | 3-1 | | | | | | |
| Sat 20/09/25 | Atherton LR v FC St Helens * | 2-1 | | | | | | |
| Tue 23/09/25 | Longridge Town v Atherton LR | 1-0 | | | | | | |
| Sat 27/09/25 | Pilkington v Atherton LR (CUP) * | 0-1 | | | | | | |
| Tue 30/09/25 | Glossop North End v Atherton LR * | 0-2 | | | |  | | |
| Sat 04/10/25 | Atherton LR v AFC Liverpool * | 3-0 | | | | | | |
| Tue 07/10/25 | Irlam v Atherton LR * | 3-0 | | | | | | |
| Sat 11/10/25 | Atherton LR v Burscough (CUP) | 1-1 | | | | | | |
| Tue 14/10/25 | Atherton LR v Wythenshawe * | 0-1 | | | | | | |
| Sat 18/10/25 | Atherton LR v West Didsbury & Chorlton * | 0-1 | | | | | | |
| Sat 25/10/25 | South Liverpool v Atherton LR | 2-1 | | | | | | |
| Sat 01/11/25 | Atherton LR v Glossop North End | 2-3 | | | | | | |
| Tue 04/11/25 | Atherton LR v Longridge Town | 3-2 | | | | | | |
| Sat 08/11/25 | Liversedge v Atherton LR (CUP) | 2-1 | | | | | | |
| Tue 11/11/25 | Atherton LR v Irlam | - | | | | | | |
| Sat 22/11/25 | Abbey Hey v Atherton LR | 1-2 | | | | | | |
