| Sat 26/07/25 | Glossop North End v City of Liverpool | 2-2 | | | | | |  |
| Tue 29/07/25 | Glossop North End v West Didsbury & Chorlton | 1-2 | | | | | | |
| Sat 02/08/25 | Glossop North End v FC St Helens (CUP) | 1-1 | | | | | | |
| Tue 05/08/25 | FC St Helens v Glossop North End (CUP) | 3-1 | | | |  | | |
| Sat 09/08/25 | FC Isle of Man v Glossop North End | 2-3 |  | | | | | |
| Tue 12/08/25 | Glossop North End v Wythenshawe | 2-2 | | | | | | |
| Sat 23/08/25 | Glossop North End v Bacup Borough (CUP) | 1-1 | | | | | | |
| Mon 25/08/25 | Abbey Hey v Glossop North End | 2-0 | | | | | | |
| Tue 07/10/25 | Glossop North End v Stockport Town * | 1-2 | | | | | | |
| Sat 11/10/25 | Pilkington v Glossop North End * | 2-0 | | | | | | |
| Sat 18/10/25 | Longridge Town v Glossop North End | 2-1 | | | | | | |
| Tue 21/10/25 | Ramsbottom United v Glossop North End | 1-0 | | | | | | |
| Sat 25/10/25 | Glossop North End v Charnock Richard | 2-0 | |  | | | | |
| Sat 01/11/25 | Atherton LR v Glossop North End | 2-3 | | | | | | |
| Tue 04/11/25 | Shirebrook Town v Glossop North End (CUP) | 0-5 | | | | | | |
| Sat 08/11/25 | Stockport Town v Glossop North End | 1-2 | | | | | | |
| Sat 15/11/25 | Burscough v Glossop North End | 1-2 | | | | | | |
| Tue 18/11/25 | Glossop North End v FC St Helens | 1-3 | | | | | | |
| Sat 22/11/25 | Prestwich Heys v Glossop North End | 0-2 | | | | | | |
| Tue 25/11/25 | Wythenshawe Town v Glossop North End (CUP) | 5-3 | | | | | | |
| Sat 29/11/25 | Euxton Villa v Glossop North End | 3-2 | | | | | | |
| Tue 02/12/25 | Glossop North End v Matlock Town (CUP) | 2-1 | | | | | | |
| Sat 06/12/25 | Glossop North End v Litherland REMYCA | 4-1 | | | | | | |
| Sat 13/12/25 | Chadderton v Glossop North End | 3-6 | | | | | | |
| Tue 16/12/25 | Glossop North End v AFC Liverpool | 1-1 | | | | | | |
| Sat 20/12/25 | Glossop North End v FC Isle of Man | 2-3 | | | | | | |
| Fri 26/12/25 | Irlam v Glossop North End | 0-1 | | | | | | |
