| Sat 26/07/25 | Cheadle Town v Barnoldswick Town | 2-0 | | | | | | |
| Tue 29/07/25 | Barnoldswick Town v Charnock Richard | 0-5 | | | | | | |
| Sat 09/08/25 | Litherland REMYCA v Barnoldswick Town | 1-3 |  | | | | |  |
| Tue 12/08/25 | Barnoldswick Town v Chadderton | 0-6 | | | | | | |
| Sat 16/08/25 | Euxton Villa v Barnoldswick Town | 2-3 | | | | | | |
| Mon 25/08/25 | Barnoldswick Town v Prestwich Heys | 2-2 | | | | | | |
| Sat 30/08/25 | Pilkington v Barnoldswick Town * | 3-3 | | | |  | | |
| Sat 06/09/25 | Wythenshawe v Barnoldswick Town | 3-0 | | | | | | |
| Sat 13/09/25 | West Didsbury & Chorlton v Barnoldswick Town | 4-0 | | | | | | |
| Tue 16/09/25 | Longridge Town v Barnoldswick Town | 1-2 | | | | | | |
| Sat 20/09/25 | Barnoldswick Town v Sunderland West End (CUP) | 10-1 | | | | | | |
| Tue 23/09/25 | Barnoldswick Town v Stockport Town | 3-3 | | | | | | |
| Sat 27/09/25 | Longridge Town v Barnoldswick Town (CUP) | 1-3 | | | | | | |
| Sat 04/10/25 | FC Isle of Man v Barnoldswick Town | 1-2 | | | | | | |
| Sat 11/10/25 | AFC Blackpool v Barnoldswick Town (CUP) | 3-0 | | | | | | |
| Tue 14/10/25 | Barnoldswick Town v Ramsbottom United | 1-3 | | | | | | |
| Sat 18/10/25 | Barnoldswick Town v AFC Liverpool (CUP) | 1-0 | |  | | | | |
| Tue 21/10/25 | Irlam v Barnoldswick Town | 1-2 | | | | | | |
| Sat 25/10/25 | Barnoldswick Town v Pilkington | 6-1 | | | | | | |
| Sat 01/11/25 | Barnoldswick Town v Burscough | 1-2 | | | | | | |
| Tue 04/11/25 | Ramsbottom United v Barnoldswick Town | 4-1 | | | | | | |
| Sat 08/11/25 | Barnoldswick Town v Winsford United (CUP) | 1-2 | | | | | | |
| Tue 11/11/25 | Barnoldswick Town v Longridge Town | - | | | | | | |
| Sat 15/11/25 | Stockport Town v Barnoldswick Town | 2-0 | | | | | | |
| Sat 22/11/25 | Barnoldswick Town v FC St Helens | 1-1 | | | | | | |
