| Sat 26/07/25 | Glossop North End v City of Liverpool * | 2-2 | | | | | | |
| Tue 29/07/25 | Glossop North End v West Didsbury & Chorlton * | 1-2 | | | | | | |
| Sat 02/08/25 | Glossop North End v FC St Helens (CUP) | 1-1 | | | | | | |
| Tue 05/08/25 | FC St Helens v Glossop North End (CUP) * | 3-1 | | | | | | |
| Sat 09/08/25 | FC Isle of Man v Glossop North End | 2-3 | | | | | | |
| Tue 12/08/25 | Glossop North End v Wythenshawe | 2-2 | | | | | | |
| Sat 23/08/25 | Glossop North End v Bacup Borough (CUP) | 1-1 | | | | | | |
| Mon 25/08/25 | Abbey Hey v Glossop North End | 2-0 | | | | | | |
| Sat 30/08/25 | Glossop North End v South Liverpool | 0-1 | | | | | | |
| Sat 06/09/25 | Glossop North End v Pilkington | 0-4 | | | | | | |
| Tue 09/09/25 | Padiham v Glossop North End | 3-1 | | | | | | |
| Sat 13/09/25 | Litherland REMYCA v Glossop North End | 1-2 | | | | | |  |
| Tue 16/09/25 | Glossop North End v Prestwich Heys | 1-2 | | | |  | | |
| Tue 23/09/25 | Cheadle Town v Glossop North End * | 0-1 | |  | | | | |
| Sat 27/09/25 | Cheadle Town v Glossop North End (CUP) * | 3-2 | | | | | | |
| Tue 30/09/25 | Glossop North End v Atherton LR * | 0-2 | | | | | | |
| Sat 04/10/25 | Glossop North End v Chadderton | 0-3 | | | | | | |
| Tue 07/10/25 | Glossop North End v Stockport Town | 1-2 | | | | | | |
| Sat 11/10/25 | Pilkington v Glossop North End | 2-0 | | | | | | |
| Sat 18/10/25 | Longridge Town v Glossop North End | 2-1 | | | | | | |
| Sat 25/10/25 | Glossop North End v Charnock Richard | 2-0 | | | | | | |
| Sat 01/11/25 | Atherton LR v Glossop North End | 2-3 |  | | | | | |
| Tue 04/11/25 | Shirebrook Town v Glossop North End (CUP) | 0-5 | | | | | | |
| Sat 08/11/25 | Stockport Town v Glossop North End | 1-2 | | | | | | |
| Sat 15/11/25 | Burscough v Glossop North End | 1-2 | | | | | | |
| Tue 18/11/25 | Glossop North End v FC St Helens | 1-3 | | | | | | |
| Sat 22/11/25 | Prestwich Heys v Glossop North End | 0-2 | | | | | | |
