| Sat 26/07/25 | Longridge Town v FC St Helens * | 2-1 | | | | | | |
| Tue 29/07/25 | FC St Helens v FC Isle of Man * | 2-0 | |  | | | | |
| Sat 02/08/25 | Glossop North End v FC St Helens (CUP) * | 1-1 | | | | | | |
| Tue 05/08/25 | FC St Helens v Glossop North End (CUP) | 3-1 | | | |  | | |
| Sat 09/08/25 | Ramsbottom United v FC St Helens * | 1-1 | | | | | | |
| Tue 12/08/25 | AFC Liverpool v FC St Helens | 1-5 | | | | | | |
| Sat 16/08/25 | FC St Helens v Atherton LR (CUP) * | 0-2 | | | | | | |
| Sat 23/08/25 | FC St Helens v Penistone Church (CUP) | 1-1 | | | | | | |
| Mon 25/08/25 | Chadderton v FC St Helens | 4-1 | | | | | | |
| Sat 30/08/25 | Burscough v FC St Helens | 1-2 | | | | | | |
| Fri 05/09/25 | Euxton Villa v FC St Helens | 1-2 | | | | | | |
| Wed 10/09/25 | South Liverpool v FC St Helens | 1-2 | | | | | | |
| Sat 13/09/25 | Abbey Hey v FC St Helens | 0-3 | | | | | |  |
| Sat 20/12/25 | FC St Helens v Cheadle Town * | 1-1 | | | | | | |
| Fri 26/12/25 | Pilkington v FC St Helens * | 3-2 | | | | | | |
| Sat 17/01/26 | Padiham v FC St Helens | 2-1 | | | | | | |
| Tue 20/01/26 | FC St Helens v City of Liverpool * | 1-0 | | | | | | |
| Sat 24/01/26 | FC St Helens v Barnoldswick Town * | 5-0 | | | | | | |
| Wed 28/01/26 | Litherland REMYCA v FC St Helens | 2-2 | | | | | | |
| Sat 31/01/26 | FC St Helens v Charnock Richard | 6-2 | | | | | | |
| Tue 24/02/26 | FC St Helens v West Didsbury & Chorlton | 3-0 | | | | | | |
| Fri 27/02/26 | FC St Helens v Burscough | 1-0 | | | | | | |
| Tue 03/03/26 | FC St Helens v Euxton Villa | 0-0 | | | | | | |
| Sat 07/03/26 | West Didsbury & Chorlton v FC St Helens | 1-1 | | | | | | |
| Sat 14/03/26 | FC St Helens v Ramsbottom United * | 1-2 | | | | | | |
| Sat 21/03/26 | FC St Helens v Longridge Town | 2-1 | | | | | | |
| Sat 28/03/26 | Prestwich Heys v FC St Helens | 1-0 | | | | | | |
| Tue 31/03/26 | FC St Helens v Atherton LR | 1-3 | | | | | | |
| Sat 04/04/26 | FC St Helens v Glossop North End | 0-0 | | | | | | |
| Mon 06/04/26 | FC St Helens v Pilkington | 3-2 |  | | | | | |
