| Sat 26/07/25 | Holker Old Boys v Nelson * | 5-0 | |  | | | | |
| Sat 09/08/25 | Holker Old Boys v Steeton * | 1-2 | | | | | | |
| Sat 23/08/25 | Holker Old Boys v Ryton & Crawcrook Albion (CUP) | 2-0 | | | | | | |
| Sat 30/08/25 | MSB Woolton v Holker Old Boys | 0-0 | | | | | | |
| Sat 06/09/25 | Holker Old Boys v Ashton Athletic | 2-0 | | | | | | |
| Sat 13/09/25 | Holker Old Boys v Ashton Town | 0-1 | | | |  | | |
| Tue 23/09/25 | Holker Old Boys v Route One Rovers (CUP) | 3-0 | | | | | | |
| Fri 26/09/25 | Euxton Villa v Holker Old Boys (CUP) | 5-0 | | | | | | |
| Sat 11/10/25 | Droylsden v Holker Old Boys | 0-1 | | | | | | |
| Tue 14/10/25 | Squires Gate v Holker Old Boys | 0-1 | | | | | | |
| Sat 18/10/25 | Wythenshawe v Holker Old Boys (CUP) | 2-2 | | | | | | |
| Sat 25/10/25 | Holker Old Boys v Maine Road * | 3-2 | | | | | | |
| Wed 29/10/25 | Bacup Borough v Holker Old Boys (CUP) * | 1-0 | | | | | | |
| Sat 08/11/25 | Albion Sports v Holker Old Boys (CUP) | 3-2 | | | | | | |
| Wed 03/12/25 | Bacup Borough v Holker Old Boys | 1-1 | | | | | | |
| Sat 20/12/25 | Nelson v Holker Old Boys | 3-1 | | | | | | |
| Fri 26/12/25 | Holker Old Boys v Garstang * | 3-0 | | | | | | |
| Sat 17/01/26 | Holker Old Boys v MSB Woolton * | 2-2 | | | | | | |
| Sat 24/01/26 | Thornton Cleveleys v Holker Old Boys * | 3-2 | | | | | | |
| Sat 31/01/26 | Daisy Hill v Holker Old Boys | 3-3 | | | | | | |
| Tue 03/02/26 | Ashton Athletic v Holker Old Boys | 1-2 |  | | | | | |
| Sat 14/02/26 | Maghull v Holker Old Boys | 2-1 | | | | | | |
| Sat 28/02/26 | AFC Blackpool v Holker Old Boys | 3-0 | | | | | | |
| Thu 05/03/26 | Holker Old Boys v Maghull * | 3-1 | | | | | | |
| Sat 14/03/26 | Holker Old Boys v Thornton Cleveleys | 3-1 | | | | | | |
| Tue 17/03/26 | Holker Old Boys v AFC Blackpool * | 2-2 | | | | | | |
| Sat 28/03/26 | Ashton Town v Holker Old Boys | 1-0 | | | | | | |
| Tue 31/03/26 | Holker Old Boys v Colne * | 3-2 | | | | | | |
| Sat 04/04/26 | Holker Old Boys v Fulwood Amateurs | 2-3 | | | | | | |
| Wed 08/04/26 | Holker Old Boys v Droylsden * | 1-4 | | | | | | |
