| Sat 26/07/25 | Longridge Town v FC St Helens | 2-1 | | | | | | |
| Tue 29/07/25 | FC St Helens v FC Isle of Man | 2-0 | |  | | |  | |
| Sat 02/08/25 | Glossop North End v FC St Helens (CUP) | 1-1 | | | |  | | |
| Sat 09/08/25 | Ramsbottom United v FC St Helens | 1-1 | | | | | |  |
| Tue 12/08/25 | AFC Liverpool v FC St Helens | 1-5 | | | | | | |
| Sat 16/08/25 | FC St Helens v Atherton LR (CUP) | 0-2 | | | | | | |
| Sat 23/08/25 | FC St Helens v Penistone Church (CUP) * | 1-1 | | | | | | |
| Mon 25/08/25 | Chadderton v FC St Helens | 4-1 | | | | | | |
| Sat 30/08/25 | Burscough v FC St Helens * | 1-2 | | | | | | |
| Fri 05/09/25 | Euxton Villa v FC St Helens * | 1-2 | | | | | | |
| Wed 10/09/25 | South Liverpool v FC St Helens | 1-2 | | | | | | |
| Sat 13/09/25 | Abbey Hey v FC St Helens | 0-3 | | | | | | |
| Tue 16/09/25 | FC St Helens v Wythenshawe | 0-1 | | | | | | |
| Sat 20/09/25 | Atherton LR v FC St Helens | 2-1 | | | | | | |
| Tue 23/09/25 | City of Liverpool v FC St Helens | 1-2 | | | | | | |
| Sat 27/09/25 | Ashton Athletic v FC St Helens (CUP) | 2-3 | | | | | | |
| Tue 07/10/25 | FC St Helens v Prestwich Heys | 0-2 | | | | | | |
| Sat 11/10/25 | FC St Helens v MSB Woolton (CUP) | 0-2 | | | | | | |
| Tue 14/10/25 | FC St Helens v Irlam | 2-2 | | | | | | |
| Sat 18/10/25 | FC Isle of Man v FC St Helens | 2-2 | | | | | | |
| Tue 21/10/25 | Wythenshawe v FC St Helens * | 1-4 | | | | | | |
| Sat 25/10/25 | FC St Helens v Stockport Town * | 1-1 | | | | | | |
| Tue 28/10/25 | Charnock Richard v FC St Helens * | 2-2 | | | | | | |
| Sat 01/11/25 | Cheadle Town v FC St Helens | 0-3 | | | | | | |
| Tue 18/11/25 | Glossop North End v FC St Helens | 1-3 | | | | | | |
| Sat 22/11/25 | Barnoldswick Town v FC St Helens | 1-1 | | | | | | |
